Invitrogen and Nonlinear Dynamics  

2D / MS Workflow


A new standard for Protein Expression Analysis

The key to understanding proteins and their behavior in biological systems lies in dramatically improving a broad and deeper analysis of the proteome. Researchers require new standards in protein expression analysis to accelerate identification and quantitation of crucial proteins involved in disease, to discover targets for therapeutic intervention, to identify and quantify biomarkers involved in toxicity and to identify proteins that lead to altered phenotypes. The combination of metabolic and chemical labeling technologies provides researchers with the flexibility to address the most difficult quantitation problems with confidence. The full potential of these powerful labeling technologies is further realized by Invitrogen's line of Solubilizers, Calbrants and Matrices that overcome common problems associated with mass spectrometry analysis of biological samples.

  • Simple/easy to use workflows compatible with one-dimensional polyacrylamide gel electrophoresis (1D-PAGE) and liquid chromatography (LC) fractionation strategies. Mass Spectrometry analysis can be performed by your local, or neighboring, core facility.

  • Confident identification and accurate quantitation of protein expression differences in cell culture, tissue or serum samples

  • Broad protein and proteome coverage including post-translational modifications (PTM), membrane proteins and biomarkers

  • Powerful data analysis software simplifies data interpretation

Invitrosol MALDI Protein Solubilizer Kit - more info
Invitromass Molecular Weight Calibrants - more info
Invitrogen’s proteomics range – more info

 

 

software evaluation
   
© Nonlinear Dynamics and Invitrogen Corporation
Hardware and reagents
1D electrophoresis
2D electrophoresis
2D/MS workflow