The intuitive and unbiased workflow of Progenesis LC-MS makes the labor-intense data evaluation much more efficient. The support of the mz-XML data format enables the analysis of datasets acquired on many different MS instruments, eliminating the dependency on specialized vendors' software.
Fast, accurate and objective result for protein quantification
We've illustrated our unique label-free analysis approach by quantifying and correctly identifying a mixture of known proteins in the 2-1,000 fmol range subjected to LC-MS/MS by an independent laboratory*. Read the full results but some data and the benefits shown by this study are below.
Peptide ion expression changes in the 2-1000fmol range were reliably detected with 83% of features producing R2 >0.8 from plotting mean raw abundance vs. injection volume.
All the known proteins of various concentrations within the mixture were correctly detected. Every one showed a linear response based on calculated protein abundance vs. injection volume with R2 values >0.9.
All nine known proteins in the mixture, as well as several contaminants (Catalase and Trypsinogen) and an additional entry for GDH (in brackets), were quantified and correctly identified by label-free LC-MS data analysis using Progenesis LC-MS.
The benefits of the workflow also provided
Speed
Complete quantification of 9 LC-MS/MS runs, ~65MB each, took 15 minutes to generate the final protein list excluding database searches. More »
Objectivity
Automatic settings were used to perform the data analysis. This approach produced identical results from three independent attempts. More »
Statistics
Peptide ion quantification was performed using objective measures within the software and the final list of interesting proteins was produced blind. More »
View other posters of Progenesis LC-MS being used to generate results in the lab.
