Evidence Progenesis SameSpots works

Here is a selection of references showing how Progenesis SameSpots delivers reliable results with speed, objectivity and statistics that can be reproduced across-labs. If you have an example from your research, get in touch, and we can share it here and on our blog.

1. Measuring 2D gel quality within and across-labs. Study involving 17 labs and Progenesis SameSpots to objectively measure how many could run 2D gels to an accepted standard. With help and feedback from the software, 100% of the labs achieved the quality threshold.
   
   
2. A multi-lab study by the ProteoRed network. Differential expression analysis by Progenesis SameSpots showed excellent within and across-lab reproducibility, >70% of spots had CV’s <20% in both cases.
   
   
3. Proving reproducibility of 2D proteomics. 5 expert proteomics labs run samples of H. influenzae ± actinonin on 2D gels and compared their lists of top 50 differentially expressed spots. Fully automatic image analysis by Progenesis SameSpots increased across-lab reproducibility compared to results that included user-editing.

1. Analysis in less than five minutes per gel. A European pharmaceutical company shelved a 20 gel experiment when the images could not be analysed in a realistic time. Progenesis SameSpots took <5 minutes per gel to generate a hit list of 30 interesting spots.
   
   
2. Helping to process thousands of 2D gels. The Biomolecular Resource Facility at The University of Texas Medical Branch saves time and money analysing hundreds of 2D gels each year using Progenesis SameSpots.
   
   
3. Analyse complex images in hours instead of weeks. The Istituto di Ricerche Farmacologiche "Mario Negri" accelerated 2D gel analysis using Progenesis SameSpots, enabling them to include more replicates per experiment and increase statistical confidence in results.
   
   

1. Get the same results whatever your analysis experience. 5 users of varying experience analysed the same set of gels using Progenesis SameSpots and results were compared to those from our in-house expert. Each users list contained >90% of the same spots as the experts list.
   
   
2. Proven objectivity and quantitative accuracy. An independent, published study showed Progenesis SameSpots outperformed other software in consistency of spot matching, as well as accurate protein loading over a 100-fold range.
   
   
3. Easy-to-use workflow with 100% spot matching. The Laboratoire d’oncopharmacologie at the Centre Régional de Lutte Contre le Cancer Paul Papin reported increased confidence in results with 100% matching using Progenesis SameSpots, and an objective workflow, easier to use than other packages.
   
   

1. Statistically valid, high quality results from 2-DE analysis. The Cardiac Proteomics group at St. George's University of London used Progenesis SameSpots to identify protein changes linked to heart disease. Using multivariate statistical tests improved the quality of a crucial experiment by highlighting an outlying sample that could be removed.
   
   
2. A structured approach to identify significant protein changes. A proteomics experiment analysed automatically and multivariate statistics applied with no prior assumptions. This reduced data complexity and found relationships that may have been missed exploring each measure independently.
   
   
3. Spot data is fully matched and shows improved noise properties. Progenesis SameSpots solves the challenge of missing values, a challenge for traditional 2D gel analysis approaches, by producing a complete data set (i.e. no missing values) regardless of how many replicates you run.