Advanced Statistical Analysis Specifications

• Multivariate statistical analysis of selected features (spots, proteins or peptides) from Progenesis SameSpots and Progenesis LC-MS

Data Table

• Calculation and display of ANOVA p-values
  
• Calculation and display of q-values for indication of False Discovery Rate (FDR)
  
• Feature(s) shown in expression profile view
  
• Selected feature(s) highlighted on Principal Components Analysis (PCA) plot
  
• Selected feature(s) highlighted on Correlation Analysis dendrogram view
  
• Calculation and display of feature power
  
• Colour coding of data in table by cluster

Feature Tags

• Colour coded tags to assist with data exploration
  
• Right click on highlighted group of features to tag
  
• Add name label to spot tag
  
• Filter spot list by tags
  
• Tag editing
  
• Features can be tagged multiple times
  
• Feature tags maintained throughout the workflow

Image View*

• Display of whole image with selected features highlighted
  
• Colour coded display of features which cluster in the correlation analysis

*Currently only available for the analysis of 2D image analysis data from Progenesis SameSpots

Principal Components Analysis

• Calculate principal components based on all features
  
• Visualisation of samples and features score and as loading plots on PCA graph with samples and features highlighted for visual confirmation of clustering
  
• Colour coded display of features which cluster in the correlation analysis
  
• Selection of principal components to display on axis
  
• Zoom functionality

Correlation Analysis

• Correlation analysis and visualisation of feature expression
  
• Visualisation of clustered features showing correlation expression profiles
  
• Hierarchical clustering of features based on expression profile data and visualisation with an interactive dendrogram
  
• Multiple selection of dendrogram nodes for expression profile comparison
  
• Multiple group selection on dendrogram by setting click and drag "pruning threshold" for expression profile comparison
  
• Zoom functionality

Power Analysis

• Post hoc, per feature power calculations
  
• Prediction of required replicates for 80% power per spot based on measured data