Creating a new experiment

To create a new experiment, click on the "New" button on the start-up screen.

Then enter the name for your experiment, and choose between:

• Single stain per gel - when each image is a separate gel, e.g. when using Sypro Ruby or Deep Purple.
• DIGE with internal standards - when you are using DIGE and want to use internal standards to improve normalisation.
• Multiple stains per gel without internal standards - when each gel is scanned multiple times, such as with DIGE, but without internal standards so the analysis will be treated as single stain.

Once you have created your experiment, add the images you want included in your experiment by clicking on "Add images" (highlighted below). This will also run each image through some quick quality control checks, to help find any potential problems (e.g. low dynamic range).

When you have added your images, click on the "Section Complete" button to continue through the Progenesis SameSpots workflow.