SameSpots enables us to apply objective statistical tests, and to visualise and organise meaningful results to achieve a clearer understanding of our data. We are finding SameSpots to be an invaluable tool in our research programmes.
Manual Alignment
If the automatic alignment can't align some of your images accurately, you can help the process by adding manual alignment vectors to those images. You can use the "Remove Vectors" button on the toolbar to clear the automatic alignment before doing any manual alignment. The manual alignment can then be used as a starting point for the automatic alignment. It is possible to align your images entirely manually, but this is time consuming and can lead to overly subjective results.
To perform manual alignment, follow these steps
- Press (and hold) the left mouse button on the green spot that you want to align.
- Drag that spot over the equivalent purple reference image spot.
- Release the mouse button to add the alignment vector.
- Repeat for any other spots you want to manually align.
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You usually only need to align a few spots manually. Once some are aligned, the automatic alignment will use those as a starting point. This is usually enough to give you a high level of alignment.
See Also: Why do I need to align my gels?
