Images with saturation

When scanning a gel, clipping can occur at either end of the range of pixel values causing the spots or the background to become saturated. This causes problems with both measuring and spot detection.

When clipping happens at the high end, the top of the spots are cut off.

This leads to incorrect quantitation, because the missing area of the spot (the top in this case) will not be measured.

With clipping at the low end of the pixel values, the bottom of the spots are cut off.

This leads to the background being measured higher than it really is, as well as some area at the bottom of the spot being unmeasured.

When an image has been clipped during scanning, there is no way to get the missing data back. The only option is to rescan the gel.