We considered 2D image analysis to be one of the big bottlenecks in 2D gel proteomics. SameSpots has completely changed that. 2D gel analysis is now easy and we do much more 2D experiments.
Using tags
Note: this page describes tagging in Progenesis SameSpots v4.0 and earlier. If you're using SameSpots v4.1 or later, please refer to the new tagging FAQ.
Tags allow you to attach a label to a selection of spots. Those labels (tags) can then used to filter the spots you're working with at each stage in the workflow
Tagging spots
To tag all the spots with an Anova p-value < 0.05:
- Sort the spot list by Anova, so the smallest (most significant) p-values are at the top
- Select all the spots with an Anova p-value less than 0.05
- Right click on the selected spots, and choose "New tag" from the menu
- Enter a name for the new tag, e.g. "Anova p < 0.05". You can also choose a colour
for the tag here by clicking the coloured box next to the name. Click OK when you
are happy with name and colour for this tag.
Filtering tagged spots
To show or hide spots based on their tags, use the drop down menu on the tag column (accessed by clicking the down arrow next to the word tag). Then choose which tags you want to show or hide, and click OK to apply the filter.
When using the tag filtering, you can include or exclude spots based on their tags. For example:
This means spots tagged with "First Tag" will be shown, all other spots will be hidden.
This means spots tagged with "Second Tag" will be hidden, all other spots will be shown.
This means all spots tagged with "First Tag" and all spots tagged with "Second Tag" will be shown, all other spots will be hidden.
This means all spots tagged "First Tag" will be shown, unless they are also tagged with "Second Tag". All other spots will be hidden.
Sell also: How do I investigate analysis results?
