Progenesis QI for proteomics FAQs
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System requirements & compatibility
- What PC specification do I need to run Progenesis QI for proteomics?
- Why is it better to run Progenesis QI for proteomics on a 64-bit operating system?
- What file formats, data types and instrument types are supported?
- What search engines and databases are supported?
- What inclusion list formats are supported?
Licensing
- What are sample licence codes and how do they work?
- How do I update my dongle to enable analysis in a new version of the software?
Quick start & overviews
Recent Experiments screen
- What are the implications of deleting a file from the Recent Experiments list?
- How can I send my experiment to a colleague or collaborator?
- What is the Progenesis Improvement Program?
- How can I backup my experiments?
New Experiment window
Import Data screen
- Which file formats are supported?
- My file format isn't listed; how can I import my data?
- What is an ion intensity map?
- Can I begin analysis while my run data is importing?
- What do the “peak count” values mean in the About This Run box?
- How can I assess the quality of my chromatography?
- How do I remove a run from my experiment?
- My runs show far fewer ions than I would expect; what might have caused this?
- Should I remove runs that have been imported with warnings?
- What are masks used for in Progenesis?
- How can I apply the same mask to all runs?
- What does it mean for runs to be “protected from editing”?
- How should I choose my alignment reference?
Review Alignment screen
- How does Progenesis correct for drifts in retention time?
- Why is alignment so important?
- What do the visualisations at Review Alignment show?
- How can I align my samples automatically?
- How should I choose my alignment reference?
- What is an alignment vector?
- How do I review and edit the alignment of my runs?
- How do I manually align runs that fail to align automatically?
- How can I correct localised alignment errors?
- How can I prevent my manual vectors snapping to the wrong location?
- How can I zoom to an area in the Review Alignment screen?
Peak Picking Parameters window
- Which runs should I use for peak picking?
- How can I detect more ions in my samples?
- How can I avoid peak-picking the contaminants, salt-front, etc. in my samples?
Review Normalisation screen
- How does normalisation work in Progenesis?
- Do I need to filter out MS1 data to control for outliers and for normalisation to work?
- How does normalisation behave where peptide ions significantly altered in their abundance are derived from proteins which do not express any changes in their abundance?
Experiment Design Setup screen
- How do my experiment designs affect analysis?
- Do I need to include all runs in each of my experiment designs?
- How do I analyse a mixture of technical and biological replicates?
- Can I import my experiment designs from an Excel file (or other file)?
Peptide & Protein Statistics screens
- What does Principal Component Analysis (PCA) show?
- What does the dendrogram show, or what is correlation analysis?
- What are p-values?
- What are q-values, and why are they important?
- What is power analysis?
Identify Peptides screen
- How can I use MSá´± data to identify my peptides?
- How do I run an MS/MS ion search?
- How can I get more identifications using inclusion lists in Progenesis?
General analysis and productivity
- How can I show only the interesting peptides or proteins?
- How can I quickly show a known location in the ion intensity maps?
- Can I use wildcards when filtering my runs and peptides?
Algorithms
- What are data compression and peak modelling and why do you do this?
- How are retention times automatically aligned across runs?
- How are peptide abundances calculated?
- How are protein abundances calculated?
- How does normalisation work in Progenesis?
- Why are protein measurements stabilised?
Fractionation workflow
- Why should I fractionate my biological samples?
- How do I analyse fractionated biological samples in Progenesis QI for proteomics?
- How (& why) should I order my fractions after importing them?
- What is the relevance of the Peptides per fraction chart?
- Why do I need to recombine my fractionated samples in Progenesis QI for proteomics?
- How do I use the Recombine Samples page to recombine my fractionated samples?
- How is normalisation performed in fractionated LC-MS experiments?