Waters | Nonlinear Dynamics

Progenesis QI for proteomics

The next generation in LC-MS proteomics data analysis software.
Discover the significantly changing proteins in your samples.


How do I use the QC metrics LC peak width chart?

This chart shows the proportional distribution of retention time peak widths in seconds, for all the detected peptide ions in the experiment (given the single aggregate detection, this is not affected by changing the experimental condition groupings). Widths are binned into columns. This chart is designed to reveal whether elution of peaks is occurring as expected for your chromatographic set-up; if the mean peak width is higher or lower than is typical for your system, you may wish to investigate the chromatography, or alter your detection settings.

Successfully identified peptide ions are shown within the bars in yellow, to allow visualisation of the proportion of peptide ions that have been successfully assigned to peptides and any correlation of this with peak width. The example demonstrates the more successful identification of peptide ions with a longer peak width, which might be expected given these are likely to be more abundant.

The LC peak width chart.

The LC peak width chart.

See also